DIG-High Prime DNA Labeling and Detection Starter Kit I Roche 11745832910
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发布时间: 2018-03-20 18:38
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货号: | 11745832910 |
供应商: | 前尘生物 |
保存条件: | -20°C |
数量: | 大量 |
英文名: | DIG-High Prime DNA Labeling and Detection Starter Kit I |
规格: | kit |
DIG-High Prime DNA Labeling and Detection Starter Kit I
Kit for color detection with NBT/BCIP.产品应用:
DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
产品介绍:
Sensitivity and specificity:The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
组分:
- DIG-High Prime, 5x conc., 50 µl
- DIG-labeled Control DNA, 20 µl, (5 µg/ml), pBR328 (linearized with Bam HI)
- DNA Dilution Buffer, 3 x 1 ml
- Anti-digoxigenin-AP Conjugate, 100 µl
- NBT/BCIP Stock Solution, conc., 6 x 1 ml
- Blocking Solution, 10x conc., 4 x 100 ml
- DIG Easy Hyb Granules, 4 x 100 ml
产地 | 产品名称 | 产品货号 | 规格 | 目录价 |
Roche | DIG-High Prime DNA Labeling and Detection Starter Kit I | 11745832910 | kit | 4534.92 促销价:3968.00 |
温馨提示:不可用于临床治疗。